Star Trek Expanded Universe
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The USS Horatio (NCC-10532) was an Ambassador-class starship in service during the mid-24th century.

Captain Jenna Steele was Horatio's first commanding officer in 2326. (Dockyard Review)

Cadet Michael Owens served on the Horatio for four months in 2354. It was while serving on Horatio that he developed his aspirations to become a starship captain. A dream which would become reality when he took command of the USS Eagle in 2371. (The Star Eagle Adventures)

In 2364, Captain Walker Keel was the Horatio's commanding officer. That year, the USS Enterprise-D detected the Horatio's wreckage in Sector 63 after meeting with the Enterprise and two other ships at Dytallix B with regards to the alien parasite. Keel suspected his first officer and chief medical officer might be under the alien parasite's control, and further investigation revealed the Horatio's destruction, including a loss of all hands, was a result of sabotage. (TNG: "Conspiracy")

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